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SRX26109955: full length 16S rRNA gene sequencing of planktonic community from the Scheldt river, Belgium; sample 47
1 OXFORD_NANOPORE (MinION) run: 33,823 spots, 65.9M bases, 58.3Mb downloads

Design: 50-100ml water sample, DNA extracted with Dneasy PowerLyzer Microbial Kit (Qiagen), full-length 16S rRNA gene amplified with 27F and 1492R bacterial primers using Phire Tissue direct PCR Master Mix (Thermo Fisher), library prepared with ONT SQK-LSK109 and sequenced on a R9.4.1 flow cell
Submitted by: Ghent University-Phycology research group
Study: CONCOMPRA validation data
show Abstracthide Abstract
Full-length 16S rRNA gene of a synthetic bacterial community, composed of evenly mixed genome DNA of 20 bacterial strains, (ATCC MSA-1002 TM), sequenced on R9 and R10 ONT MinION flowcells
Sample: freshwater-brackish water microbiome
SAMN43801086 • SRS22674265 • All experiments • All runs
Library:
Name: 16S_nat_com_47
Instrument: MinION
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 33,823 spots, 65.9M bases, 58.3Mb
Run# of Spots# of BasesSizePublished
SRR3069230333,82365.9M58.3Mb2024-09-17

ID:
35227360

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